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KMID : 1007020100080020171
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Korean Soceity of Osteroporosis
2010 Volume.8 No. 2 p.171 ~ p.177
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The Ability of Cyclosporine (CsA) to Induce Oxigen Free Radicals in a Rat Osteoblast Cell Line
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Jung Min-Hyung
Choi Woong-Hwan
Lee Tae-Hwa
Oh Young-Rim
Kim Heung-Yeol
Lee Ji-Young
Yang Seung-Oh
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Abstract
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Objectives: This study examined the ability of Cyclosporine (CsA) to induce apoptosis in a rat osteoblast cell line.
Methods: Rat osteoblast ROS 17/2.8 cells were cultured, and treated with with 0.1～40μg/mL CsA for 24 hours after plating of cells. Cell viability was determined by the MTT assay. Western Blot Analysis was done with primary antibodies to caspase-3 and caspase-8. Reactive oxygen species (ROS) synthesis was measured by flowcytometry.
Results: Cell viability decreased in dose-dependent manner with increasing concentrations of CsA. Treatment of ROS 17/2.8 cells with 0.1, 0.5, 1, 5, 10, 20, or 40 μg/mLg/mL CsA caused 85%, 80%, 73%, 60%, 45%, 40%, and 27% cell viability, respectively. Western blot analysis showed reduced caspase-3 expression and induced caspase-8. The ROS in a dose-and time-dependent manner were increased by CsA.
Conclusion: These results suggest that CsA can induce oxygen free radicals which appears to trigger apoptosis by activating pro-apoptotic signals. CsA plays a role in the post- transplatation bone diseases via the induction of apoptosis in osteoblast.
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KEYWORD
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Cyclosporine, Osteoblast, Apoptosis, Osteopenia
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